Abstract: To achieve the "carbon peaking and carbon neutrality" goal, large-scale energy bases with wind-photovoltaic power as the mainstay and thermal power unit coupled with energy storage system as primary regulation resources are being scaled up. To tackle the challenge of co-optimizing economic efficiency and frequency stability in energy base operation, a double-layer coordination economic and real-time dispatching strategy consisting of an upper-layer economic dispatch model and a lower-layer real-time frequency regulation model was proposed. The upper-layer economic dispatch model aims for optimal economic performance and provides optimal operational references to the lower-layer model. The lower-layer real-time frequency regulation model targets system frequency stability, dynamically adjusts power outputs, and tracks the upper-layer references, thus enhancing economy of operation. The effectiveness of the proposed strategy was validated by simulation experiments. Experiment results demonstrate that by adopting the proposed strategy, the energy base can obtain an average frequency deviation of 0.013 4 Hz and a maximum deviation of 0.196 5 Hz during operation, significantly improving the frequency performance of system. Meanwhile, the upper-layer model can ensure the state of charge of battery energy storage system to remain within appropriate boundaries during real-time dispatch, avoiding regulation capability loss caused by boundary violations, thereby enhancing the overall operational benefits of energy base.