Abstract: In order to verify the feasibility of ocean thermal energy conversion and test the working performance of the key equipments, test the performance of key equipment and provide reliable data support, a 50 kW ocean thermal energy power generation test platform was designed and constructed. A mathematical model of the organic Rankine cycle system was established to study the effect of temperature of cold and warm seawater on system performance. The accuracy of the theoretical design model was verified and the operating characteristics of the plant under design condition were tested. Results show that larger temperature difference enables higher thermal efficiency. The experimental results agree well with the simulation data, and the maximum power output of the system can reach 43.9 kW, with an average thermal cycle efficiency of 2.49%.